Product Info Summary
SKU: | PB9183 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GAD67/GAD1 Antibody Picoband™
SKU/Catalog Number
PB9183
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GAD67/GAD1 Antibody Picoband™ catalog # PB9183. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAD67/GAD1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9183)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GAD67 recombinant protein (Position: N14-D122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9183 is reactive to GAD1 in Human, Mouse, Rat
Applications
PB9183 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
67 kDa
Calculated molecular weight
66.897kDa
Background of GAD1
Glutamate decarboxylase 1 (brain, 67kDa) (GAD67), also known as GAD1, is a human gene. It is mapped to 2q31.1. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GAD67/GAD1 using anti-GAD67/GAD1 antibody (PB9183).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD67/GAD1 antigen affinity purified polyclonal antibody (Catalog # PB9183) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAD67/GAD1 at approximately 67 kDa. The expected band size for GAD67/GAD1 is at 67 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GAD1 (Source: Uniprot.org, NCBI)
Gene Name
GAD1
Full Name
Glutamate decarboxylase 1
Weight
66.897kDa
Superfamily
group II decarboxylase family
Alternative Names
67kD); EC 4.1.1; FLJ45882; GAD1; GAD25; GAD67; glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 67 kDa isoform GAD1 CPSQ1, DEE89, GAD, SCP glutamate decarboxylase 1 glutamate decarboxylase 1|67 kDa glutamic acid decarboxylase|GAD-67|glutamate decarboxylase 1 (brain, 67kDa)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAD1, check out the GAD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAD67/GAD1 Antibody Picoband™ (PB9183)
Hello CJ!
PB9183 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Excitatory and inhibitory neurons in the hippocampus exhibit molecularly distinct large dense core vesicles
Dietary Restriction Affects Neuronal Response Property and GABA Synthesis in the Primary Visual Cortex
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GAD67/GAD1 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-GAD67/GAD1 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-GAD67/GAD1 Antibody Picoband™
Question
We are currently using anti-GAD67/GAD1 antibody PB9183 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The anti-GAD67/GAD1 antibody (PB9183) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
I see that the anti-GAD67/GAD1 antibody PB9183 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-11
Question
My question regarding product PB9183, anti-GAD67/GAD1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9183 anti-GAD67/GAD1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-15
Question
Is this PB9183 anti-GAD67/GAD1 antibody reactive to the isotypes of GAD1?
Verified Customer
Verified customer
Asked: 2017-12-22
Answer
The immunogen of PB9183 anti-GAD67/GAD1 antibody is E.coli-derived human GAD67 recombinant protein (Position: N14-D122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-22